The present study was aimed to formulate and evaluate traumatic ulcer in the cheek mucosa of rats using methanol extract of Mangifera indica (MEMI) buccal filim. M.indica leaves were extracted with methanol and were formulated by using the water soluble ingredients (HPMC, Gelatin, Chitosan, SaCMC, PVP, PVA, and Pectin). MEMI containing F6 was found to an optimized formulation and was subjected to preliminary evaluation. A total of 24 adult male rats grouped as four, six in each Group 1- ulcer control, Group 2- Standard (Triamcinolone 0.1% ointment), Group 3- received 200mg/kg of MEMI buccal film, Group 4- received 400mg/kg of MEMl buccal film for 10 days. Ulceration of the left cheek mucosa was provoked by abrasion using scalpel blade. Body weight and their ulcers were measured with a 0.5-mm precision digital paquimeter. At the end of the study, check mucosa containing the induced ulcer was collected and given for histopathological study. Optimized formulation (F6) showed the maximum release within 6 hrs. The ulcerated area, body weight and percentage of wound contraction were significantly (p<0.001) normalized as that of normal rats. The proposed cheek mucosa ulcer model in rats can be considered an efficient process that offers reliable, reproducibility, and a low cost. Anti-ulcer herbal buccal film thus prepared by simple, low cost, nontoxic and eco-friendly method using the methanol leaf extract of M. indica. So it proved that this leaf extract can be further developed as alternative for currently used antibacterial and antiulcer agent.