Oral controlled release dosage forms have been developed over the past three decades due to their considerable therapeutic advantages such as ease of administration, patient compliance and flexibility in formulation. The objective in designing a controlled release system is to deliver the drug at a rate necessary to achieve and maintain a constant drug blood level. Aim of the study is to formulate Cytarabine based microspheres using different polymers by solvent evaporation technique. Six batches of formulations, of drug: polymer ratio i.e. 1:1,1:2 and 1:3, were prepared and evaluated for particle size, % yield, drug content, drug entrapment efficiency, Swelling studies, In-vitro mucoadhes.ion test, in vitro drug release and Flow property had shown satisfactory results. The IR Spectra’s revealed that, there is no interaction between polymer and Cytarabine. The polymer used is compatible with the Cytarabine. The basis of release data and graphical analysis formulation C3 showed a good sustained release profile with maximum entrapment efficiency because of high polymer concentration. Hence, from all the above obtained data it can be summarized that it is possible to formulate a promising sustained release mucoadhecive microspheres of Cytarabine by solvent evaporation technique using an ideal polymer like HPMC15cps +Carbopol 934p.