In this study, the antidiabetic drug repaglinide was formulated as a gastroretentive mucoadhesive tablet to increase its bioavailability. Hydroxypropyl methylcellulose K200M, carboxymethylcellulose sodium, carbomer 974P, karaya gum, chitosan and xanthan gum are used as mucoadhesive polymers in the tablet formulation. Various formulations have been prepared using different polymer concentrations. The angle repose, bulk density, tap density, Carr index and Hausner ratio of the pre-pressed mixture of repaglinide mucoadhesive tablets were characterized. The results showed that all formulations had a good swelling index. Flotation delay time and buoyancy studies showed that the formulation had good buoyancy. Drug release studies have shown that there is a controlled and improved drug release within 12 hours. In vivo studies have been conducted using well-known drugs. Based on in vitro drug release and related tests, the RT11 formulation with a drug:karaya gum ratio of 1:2 was optimized. The drug release of the RT11 formulation followed the Higuchi model with a regression value of 0.984.