Background: Herbal medicine is the sign of modern medicine and drug development. In this study we use two herbal drug as an active pharmaceutical ingredient first is an Azardichata indica second one is Occimum sanctum i.e. neem and tulsi respectively. Using this two active ingredient, we formulated anti-microbial herbal topical preparation. Materials and Methods: In this formulation, neem oil and tulsi soft extract used as anti-microbial agent and honey, camphor, lemon juice, bees wax used as pharmaceutical excipient which act as anti-oxidant, penetrating agent, pH modifier, ointment base respectively. Topical preparation was formulated using hot diffusion method. The anti-microbial topical preparation was evaluated for various evaluation parameters which include colour, odor, pH, viscosity, spredablility, homogeneity, texture, after feel, removal study, type of smear, stability studies and in-vitro anti-microbial stability.