The present study aims to investigate the potential of a transfersomal gel formulation loaded with ketoconazole as an innovative approach for treating fungal infections, offering a viable alternative to conventional dosage forms. Through optimization, the developed formulation exhibits the ability to augment the bioavailability and skin permeation of ketoconazole, thereby enhancing its therapeutic efficacy. Materials and method: ketoconazole-loaded transfersomal gel were prepared by the film- hydration method using carbopol, Tween 80, distilled water, Span 80, phosphatidylcholine, propylene glycol, soya phosphatidyl choline. Results and discussion: The optimized ketoconazole-loaded transfersom was used to prepare ketoconazole-loaded transfersomal gel with the aid of Carbopol 940 as the gelling agent. The Optimized ketoconazole-loaded transfersomal gel had a spherical shape with the particle size of, polydispersity index, the zeta potential, encapsulation efficiency, and release efficiency. In-vivo release studies showed that the cumulative amount of ketoconazole-loaded transfersomal gel was significantly higher and good bioavailability.