The main objective of present work was to formulate and evaluate sustained release matrix tablet of lamivudine using different polymers viz. Xanthan Gum, Ethyl Cellulose. Lamivudine an Antiretroviral agent which comes under BCS class III which has high solubility and low permeability, was chosen for the study. Formulation of matrix tablets was prepared by using powder blend of different ratios of polymer to get desirable drug release profile. Direct compression method was used to formulate tablets. The evaluation of physical properties of tablet were done, the in-vitro drug release study was performed in 0.1 N HCL for 2 hours and in phosphate buffer PH 6.8 up to 10 hours. Evaluation parameters of formulated matrix tablets were hardness, friability, thickness, drug content, weight variation, and the in-vitro drug release rate pattern results indicated that the formulation F3 was the most promising formulation as the drug release from this formulation was high as compared to other formulations. In formulation F3, percentage drug release of lamivudine sustained release was 98.81±0.63.