Traditional medicine systems like Ayurveda, Traditional Chinese Medicine, and Native American medicine have used natural resources for healing purposes for thousands of years. These systems have provided a foundation for modern pharmacology. Many modern pharmaceutical drugs have been developed from compounds initially isolated from plants. The Earth's biodiversity offers a vast array of potential medicinal compounds. However, a significant portion of these natural resources remains unexplored. This highlights the need for continued research into the pharmacognostic and pharmacological properties of various plants and organisms. The study aimed to evaluate anti-inflammatory activity and phytochemical screening of novel polyherbal formulations Tridax procumbens and Azadirachta indica. It was observed that Test group Seven had an ethanolic extract of Tridax procumbens and Azadirachta indica. (75:25) shows the best result like the standard drug Diclofenac Sodium. Test groups show dose-dependent therapeutic efficacy. Efficacy increases with increasing the dose of test formulations.