Nanoparticles (NPs) have emerged as a promising strategy for delivering drugs to the brain by bypassing the blood-brain barrier (BBB) without resorting to chemical modifications. In this study, Emulsification PEG-PLGA nanoparticles loaded with the drug were formulated using the solvent evaporation method. To achieve this, PEG-PLGA was dissolved in acetone (50 mL), and PLGA was dissolved in PEG-PLGA (50:50, 7-11 mg/mL). Chitosan-Avastin conjugates were synthesized in accordance with established procedures. Initially, Chitosan (3.25 mmol) was hydrated in 1 N HCl, followed by the addition of the appropriate amount of distilled water. Subsequently, NaOH (5N) was introduced to the mixture to adjust the pH to 5, and Avastin (6.48 mmol) was added with continuous stirring. In vitro studies aimed to assess the impact of different drugs on various cancer cell lines. A diverse range of human cancer cell lines were screened for testing purposes. Cells were cultured in 96-well plates, and the rate of cell proliferation, indicative of cell growth, was indirectly measured by the color intensity of the dye, which is inversely proportional to the number of cells present. The MTT test was conducted using doses ranging from 10 to 100 g/mL to evaluate the cytotoxicity of various polymer ratios and formulations on human lung cancer lines (A549). The percentage of viability for different samples was determined by comparing with untreated cancer cells serving as the control.