This study focused on the formulation, development, and in vitro evaluation of hydrogels containing Saraca Indica bark extract for potential antimicrobial application. Three different hydrogel formulations were prepared using different polymers: Carbopol 934 (F1), Carbopol 940 (F2), and HPMC (F3). The hydrogels' organoleptic properties, physical attributes, pH, viscosity, spreadability, drug content, and in vitro drug release were investigated. Results revealed favorable organoleptic and physical properties for all formulations, with pH values compatible for skin application. Variations in viscosity, spreadability, and drug content across the formulations were observed, likely due to differences in polymer properties. Phytochemical screening indicated the presence of several bioactive compounds, including alkaloids, flavonoids, glycosides, phenols, saponins, steroids, tannins, reducing sugars, carbohydrates, and amino acids in the extract, affirming its therapeutic potential. The in vitro drug release study demonstrated the highest release rate in the F1 formulation. The findings suggested that Saraca Indica bark extract hydrogels could serve as a promising antimicrobial treatment. However, further in vivo experiments and clinical trials are required to establish their real-world efficacy and safety.