Acyclovir is an antiviral drug widely used for the treatment of various viral infections, including herpes simplex and varicella-zoster viruses. However, its short half-life and frequent dosing regimen pose challenges in maintaining consistent therapeutic levels. To overcome these limitations, this study aimed to develop a novel Acyclovir tablet with an innovative design of an outer immediate-release layer and an inner sustained-release layer utilizing a floating tablet approach. The formulation process involved selecting appropriate excipients, optimizing drug loading, and establishing suitable manufacturing techniques. The immediate-release layer was designed to provide an initial burst of Acyclovir for immediate therapeutic effect, while the sustained-release layer was intended to maintain a prolonged release profile, ensuring a sustained drug release over an extended period. In-vitro evaluation of the developed Acyclovir tablet included various tests, such as weight variation, hardness, and friability, disintegration, and drug release studies. These evaluations were performed to assess the tablet's physicochemical characteristics, mechanical strength, disintegration time, and drug release profiles.