The potential of using the intact skin as port of drug Administration has been recognized for several decades. Transdermal therapeutic systems have been designed to provide controlled continuous delivery of drugs via the skin to the systemic circulation. Griseofulvin (GSF) loaded polyvinylpyrrolidone (PVP k-90) composite electro spun nanofiber (NF) was developed, and hybridized as a transdermal patch. The Formulation was optimized by factorial design (design expert software). There are 8 batches are prepared and evaluated. The nanofiber was prepared by using 0.56% w/w PVP and loaded with 3 % wt. GSF. The prepared NF was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), ultraviolet–visible spectroscopy, differential scanning calorimetry (DSC), and X-ray diffraction (XRD). The NF mat was hybridized in to transdermal patches then its physicalchemical parameters and in vitro diffusion of GSF were also evaluated. The prepared formulation ideally followed the Zero-order kinetics (r2 = 0.973).) considered very high and fall under the accepted range. The Optimized formulation containing diameter of nanofiber 84.03 nm Cumulative drug release of NF is 88.3% for 5 hr.