In recent years, there has been a revolutionary shift in how infectious disease management and immunization procedures are carried out. Biotechnology and genetic engineering have advanced, an emphasis has been made on the effective delivery of these biologicals, in addition to the creation of a large number of drugs targeted at certain ailments. Vesicles make up niosomes produced with cationic surfactants that are degradable, safer, stronger, and less expensive than liposomes. With a focus on their application in medicine, this text explores the present expansion of curiosity niosomes of curiosity across a number of scientific domains. A basic overview of niosome preparation techniques, types, characterization, and applications is also provided in this article.