An attempt was made to induce variability in Jasminum sambac by treating its rooted cuttings with different doses of colchicine(i.e.,0.25, 0.50, 0.75 and1 per cent). Genetic variability studies revealed that high heritability with high genetic advance as per cent of mean were registered in the traits including plant height,number of secondary branches, number of floweringcymes per plant, number of leaves, leaf thickness, leaf area and diameter of open flower which indicates that the characters are highly heritable and least influenced by environment, selection for improvement of such traits may be useful and effective. Correlation studies revealed that stem girth was highly significant and positively correlated with leaf thickness. Duncan’s multiple ranges test showed that the traits namely plant height, stem girth, number of leaves, leaf area, leaf thickness, number of flowering cymes per plant, flower bud length, corolla tube length, single flower bud weight and hundred flower bud weight expressed significance for 0.75% colchicine treatment.