Background: Genetics plays a vital role in healthcare, impacting the diagnosis, treatment, and prevention of diseases. Nurses are essential members of the healthcare team and their understanding of genetics is crucial for providing optimal care. However, the knowledge and perception of genetics healthcare among nurses in Saudi Arabia are not well understood. Aim: The aim of this study was to assess the knowledge and perception of genetics healthcare among nurses in Saudi Arabia. The study sought to gather insights that could inform strategic interventions for improving genetics healthcare among nurses. Methods: The study sought to investigate nurses' knowledge and perception of genetics healthcare in various healthcare settings in Saudi Arabia. Cross-sectional survey was conducted among the nurses. A structured questionnaire which had sections on basic genetics concepts, genetic testing, genetic counseling, and ethical considerations in genetics healthcare, was used to collect the required information. Results: The study involved 352 participating nurses. The findings revealed a moderate level of knowledge regarding genetics healthcare among nurses in Saudi Arabia, with variations in understanding across different topics. Conclusion: The study highlighted the pressing need for more education and training in genetics healthcare among nurses. It is crucial to enhance nurses' awareness of genetic testing, genetic counseling, and ethical considerations to ensure high-quality patient care. Recommendations: Prioritizing genetics education and training programs for nurses in Saudi Arabia. Workshops, seminars, and continuous professional development opportunities should be made available to ensure a comprehensive understanding of genetics healthcare. Collaboration with geneticists and counselors can create a meaningful impact through interdisciplinary teamwork, ultimately improving patient outcomes.