Green nanocomposites, a cutting-edge class of materials created by fusing renewable biopolymers with nanoscale reinforcements, show enormous potential for revolutionising biomedical applications due to their sustainable and eco-friendly qualities. The enhanced characteristics, biocompatibility, and biodegradability of these nanocomposites make them appropriate for a variety of biomedical applications. The uses of green nanocomposites in tissue engineering, biosensors, and drug delivery systems are covered in detail in this work. Green nanocomposites have the potential to improve drug delivery, safeguard fragile pharmaceuticals, and enable site-specific and tailored therapy. They are the perfect scaffolds for tissue regeneration in tissue engineering because they have certain mechanical properties that encourage cell attachment and proliferation. Green nanocomposites also provide a flexible framework for the development of sensitive and focused biosensors for the detection of biomolecules with enhanced stability and robustness. Despite these advantages, issues with scalability, biocompatibility, and regulatory approval must be resolved in order to guarantee safe and efficient clinical application. In assessing the toxicity and effect of green nanocomposites, the research also emphasises the significance of safety and environmental factors.