Fusariumoxysporum f. sp. lycopersici is responsible for causing tomato wilt disease worldwide. The use of chemical fungicides has significant negative effects on the environment and poses hazards to non-target organisms. Plant metabolites and plant-based pesticides have emerged as promising alternatives due to their lower environmental impact and reduced risk to consumers compared to synthetic pesticides. In this study, extracts from Daturastramonium L. and Calotropisgigantea L. were tested in vitro for their antifungal properties against Fusariumoxysporum f. sp. lycopersici, aiming to develop eco-friendly management strategies.