This research outlines an environmentally friendly method for producing gold nanoparticles (AuNPs) that uses Citrus medica extract as a reducing and stabilising agent. The UV-Vis spectra showed a clear absorption peak at 540 nm, which was used to confirm the production of AuNPs. The synthesisedAuNPs' spherical, 18 nm-average size was measured using transmission electron microscopy (TEM). It was feasible to confirm the existence of functional groups that were in charge of the reduction and stabilisation of AuNPs using Fourier transform infrared spectroscopy (FTIR). Four bacterial strains—Staphlococcusaureus, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi—were employed to test the antibacterial effectiveness of the synthesisedAuNPs. Overall, the results of this study indicate that the synthesis of AuNPs with strong antibacterial activity can be achieved using Citrus medica extract in a practical and environmentally friendly manner.In this study gold nano particles of Citrus medicaextract showed antibacterial activity