An aqueous extract of Tabebuia rosea has been employed to synthesize iron nanoparticles (FeNPs) by engaging as a reducing agent. IONPs were characterized using UV-spectrophotometer, FTIR, SEM, and EDX Analysis. Fourier transform infrared spectroscopy (FTIR) probed FeNPs exhibited peaks at 2854 and 2926, respectively corresponding to the following C-H stretches, 2850 cm1 (C-H of CH3, CH2, and CH, 2150-2300 cm). A change in color signaled the reaction or the development of dark indicated the existence of FeNPs. By using Blue light analysis, the greatest absorption peak (max) was identified to be at 395 nm. SEM images have been utilized to observe FeNPs with widths spanning 10 and 100 nm. Fe2O3-NPs have shown significant antimicrobial activity against Gram-positive bacteria B. subtilis, with a zone of inhibition of 30 mm, However, Gram-negative strains E. coli and P. aeruginosa with a zone of inhibition of 30 mm and 32 mm respectively. Similarly, Fe2O3-NPs showed substantial good susceptibility towards the tested fungal strain Aspergillus niger and Aspergillus flavus with the zone of inhibition 14 mm and 20 mm respectively for Fe2O3-NPs compared to 12 mm for fluconazole (p < 0:05). Antioxidant activity of various concentrations of IONPs were used (100–500 μg/ml). At the highest concentration (500 μg/ml), IONPs showed a potent DPPH radical scavenging of 89.92%, while standard ascorbic acid showed 91.82% antioxidant activity.