Silver nanoparticles (Ag-NPs) have been created using a variety of techniques, however because of their enormous size and little surface area, their catalytic activity has been reduced. The production of Ag-NPs by fungi has thus been detailed here. The created nanoparticles were determined to be 40.6 nm when the synthesised silver nanoparticles were further examined using SEM-EDS, XRD and UV-Vis spectroscopy to evaluate the size of the particles, surface, crystal structures, and phase formation of nanoparticles. The antibacterial activity of synthesized nanoparticles was examined on various bacteria including S. aureus, B. subtilis, K. pneumoniae and C. glutamicum. Silver nanoparticles are a fantastic class of wide-ranging antibacterial agent. More notably, Aspergillus niger-produced Ag-NPs showed strong antibacterial efficacy against certain infections