In the present study, green synthesized titanium dioxide nanoparticles were used to nanoprime tomato seeds to study the enhance- ment of soil parameters. Spectroscopic analysis of nanoparticles was conducted to assess the crystallite structure, particle size, morphology and polymorphism. X-ray diffraction analysis showed an intense peak at 25.27◦ which corresponds to the (101) plane and confirmed the formation of anatase polymorph having tetragonal structure of TiO2 NPs. Average crystallite size of titanium dioxide nanoparticles was calculated using Scherer’s formula and found as 59.6 nm. FE-SEM showed a mix of cuboidal and irregular shapes of the NPs. Energy bandgap was found to be 3.26 eV using UV-Vis spectroscopy which shows the formation of anatase phase. Seed germination indices of the treated seeds were higher than the controlled seeds, and this confirmed the role of titanium dioxide nanoparticles in the formation of reactive oxygen species that formed nanopores on the testa and enhanced the imbibition. Reactive oxygen species, enhanced role of gibberellic acid responsible for the seed germination and availability of nitrogen for absorption in the soil, resulting in enhancement of chlorophyll and rate of photosynthesis leading to increased nutrient uptake, biomass and crop yield.