Glycine and cyanoacetic acid were combined in a 1:1 ratio to grow Glycinium Cyanoacetate (GCA) crystals, which were then slowly grown as single crystals in methanol as a solvent. Unit cell attributes were discovered via single-crystal X-ray diffraction. Functional groups were found in GCA using FTIR spectra. The linear optical properties of solution-grown GCA crystals were studied in UV-Vis spectrometers. Vickers Microhardness Tester was used to assess the mechanical properties of the crystal. TG/DTA study was carried out to find the thermal stability and water of crystallization. The transmittance and absorbance behaviour of the sample has been tested by UV-visible spectral studies The LDT value of GCA crystal was found by using Nd: YAG laser The antibacterial properties of the GCA were evaluated using the Agar disc diffusion method.