liver disease has emerged as a major cause of mortality in patients with B- thalassemia major (TM). Objective: To assess the possible role of iron overload as a cause of liver dysfunction in thalassemic children receiving multiple blood transfusions and its correlation with serum aminotransferases. Patients and methods: The present study was a one year descriptive cross-sectional study aimed to assess the possible role of iron overload as a cause of liver dysfunction in thalassemic children receiving multiple blood transfusions and its correlation with serum aminotransferases in children who attended to the Thalassemic Center at Hematological Department of Assiut University Children Hospital in the period from the first of January 2021 up to the end of December 2022. The study included 79 thalassemic children. Results: In the current study showed that Statistically significant negative correlation was observed between the hemoglobin level & hematocrit (%) and the iron profile of the studied cases. Also, all studied iron profile showed statistically significant positive correlations with liver enzymes (ALT & AST levels). our study demonstrated that albumin synthesis decreased as the severity of iron overload increased. The coefficient correlation between iron profile and albumin was negative, unlike the transaminases. Another significant positive correlation was observed between iron profile of the studied cases and bilirubin level. Conclusion: This study demonstrates that most children with thalassemia have iron overload. It was inferred that iron overload is a principle reason for raised liver enzymes (ALT, and AST), decreased albumin level, and increased bilirubin level.