This study investigated the hepatoprotective potential of Eggelepanth skin ethanol extract (ESEE) at three different doses (200, 400, and 600 mg/kg) against Aflatoxin B1 (AFB1)-induced liver injury in rats, with a focus on the markers AST, ALT, ALP, and GGT. The rats were randomly divided into ten groups: control, AFB1 only, and four ES-EE treatment groups. After four weeks of treatment, the liver markers were measured, and liver tissues were analyzed histologically. The results showed that AFB1 caused a significant increase in the levels of AST, ALT, ALP, and GGT compared to the control group. However, treatment with ES-EE significantly reduced the levels of these markers in a dose-dependent manner. Additionally, histological analysis revealed that the extract prevented liver damage induced by AFB1, as evidenced by reduced necrosis and inflammation. In conclusion, Eggelepanth skin ethanol extract at doses of 200, 400, and 600 mg/kg exhibited significant hepatoprotective potential against AFB1-induced liver injury in rats, as indicated by the reduced levels of AST, ALT, ALP, and GGT