Diplazium esculentum is an edible fern found in Himalayas. Its fronds are traditionally used in headache, pain, fever, wounds, dysentery, glandular swellings, diarrhoea and various skin infections. This study investigated the antioxidant, anti-inflammatory, antimicrobial and cytotoxic properties of the D. esculentum young fronds using different in vitro experimental models and detection of phytochemicals present. The results of the present study suggest that the leaf of D. esculentum possess significant cytotoxic, antimicrobial and antioxidant properties and recommend its use as a chemoprotective dietary supplement, however further in-depth studies needed to elucidate its mode of action as well as its toxicity profile.