Critically ill children frequently require central lines [central venous catheter (CVC) and artery line). CVCs are used for various reasons such as inotropes, total parenteral nutrition, renal replacement therapies, plasma exchange, blood sampling and invasive monitoring. Despite the many benefits of CVCs, they carry significant risks such as central line associated blood stream infection (CLABSI) and thrombosis. CLABSI is one of the worst types of HAIs, with a mortality rate of 12%-25%. Aim: The aim of this study was to determine the incidence of CLABSI, the causative organism, patient’s risk factors and their impact on hospital stay length, morbidity and mortality. This will help the healthcare providers for better control of infection in children with conditions necessitating central line catheterization. Methods: We enrolled children aged from 1 day to 18 years who were admitted to Mansoura University Children’s Hospital, with central lines inserted at hospital, during the period from March 2021 to March 2023. Central and peripheral blood cultures were done for each patient, after 48 hours of central line insertion and repeated whenever infection was suspected. Results: A total of 68 children, with CLs inserted in MUCH, their median age 3 years ,57.4% of them were males,42.6% were females, were evaluated. From those 46 patients developed CLABSI. The study revealed the most common pathogen responsible for development of CLABSI in MUCH is staphylococcus aureus followed by klebseilla and candida. Conclusion: Based on the findings of the current study, it can be concluded that CLABSI is common among pediatric especially during infancy. Staphylococcus aureus followed by klebseilla are common pathogens causing CLABSI in MUCH. Jugular site of CVC insertion associated with lower incidence of CLABSI compared to femoral. CLABSI associated with high morbidity and mortality among patients especially those admitted in ICU and NICU