AIM- The aim of the present investigation is to study the HPTLC Finger print Analysis of Ethyl Acetate & Effect of Different Extracts of Leaves & Flowers of Rungia repens Nees on Lipid Levels in Diabetic Rats. MATERIAL & METHODS- After fasting 18 hours, the rats were injected intraperitoneal injection through tail vein with a single dose of 40 mg/kg Streptozocin (Sigma, St. Louis, Mo, USA), freshly dissolved in citrate buffer (pH 4.5). After injection, the rats had free access to food and water and were given 5% glucose solution to drink overnight to counter hypoglycemic shock. Then serum samples were also used to analyze for serum Total Cholesterol (TC), Triglycerides (TG), High Density Lipoprotein Cholesterol (HDL-C), Low Density Lipoprotein Cholesterol (LDL-C) and Very Low Density Lipoprotein Cholesterol (VLDL-C). HPTLC fingerprint profile was established for ethyl acetate extract of Rungia repens Nees. One side of twin trough chamber was charged with the solvent system petroleum ether: ethyl acetate (66:34) for ethyl acetate extracts and allowed to equilibrate for 10 minutes. Plate was then scanned at 254 and 366 nm in case of ethyl acetate extract. RESULTS- On the treatment of methanolic extract of leaves showed a very good effect on lipid profile. It showed highly significant (p<0.001) effect on lipid profile in comparison to that of diabetic group. Ethyl acetate extract of barks showed a very good effect on lipid profile. It showed highly significant (p<0.001) effect on lipid profile in comparison to that of diabetic group. Ethyl acetate extract also showed a highly significant effect on various lipids and also increased HDL level as compared to disease group or diabetic animals. CONCLUSION- In present study, the methanolic extract of flowers and ethyl acetate extract of produced a marked decrease in blood glucose levels at 200 mg/kg and 400 mg/kg body weight in streptozotocin-diabetic rats after 21 days treatment.