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ISSN 2063-5346
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HYPOTHESIZING THE USE OF AYURVEDIC PREPARATION (PRAVAL BHASMA) TO REPLACE STANDARD OSTEOGENIC BONE COCKTAIL FOR INDUCING OSTEOGENIC DIFFERENTIATION IN HUMAN DENTAL PULP STEM CELLS (HDPSCS).

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Dr Uday Londhe, Dr. Kalyani Bhate, Dr Ramesh Bhonde, Dr Lakshmi Shetty, Avinash Kharat, Vd Arati Dubewar, Dr Deepak Kulkarni
» doi: 10.31838/ecb/2023.12.6.164

Abstract

Introduction: The treatment of bone defects is a major problem in Dentistry. Regeneration of bone can be done using stem cell therapy. Stem cells can be derived from Dental Pulp (hDPSc). hDPSc when exposed to Standard Osteogenic Bone cocktail (Dexamethasone, Ascorbic acid and beta glycerophosphate ) differentiate into osteoblast and deposit bone. Except for Ascorbic Acid, Dexamethasone and glycerophosphate have little osteogenic potential. Hypotheses: Human Dental Pulp Stem Cells (hDPSCs) when preconditioned with Praval Bhasma induce osteogenic potential in Human Dental Pulp Stem Cells (hDPSCs). Evaluation Of Hypotheses: The Human Dental Pulp Stem Cells (hDPSCs) will be preconditioned using Praval Bhasma as a constituent of Standard Osteogenic Bone Cocktail by replacing one ingredient with Praval Bhasma.The cell culture will be incubated at 37 degrees celcius and evaluated histologically for evaluation of bone deposition

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