Introduction: Poor oral hygiene is one of the major causes oforal infections and its related pathologies in the general population. Notwithstanding seemingly appropriate treatment, extreme orofacial infections can advance and lead to life-threatening complications. Another crucial concern is about the drug’s sensitivity and resistance to oral microflora.This study was accomplished to evaluate the drug’s sensitivity of microbial isolates from the oral cavity in hospitals of Odisha. Methods: In this study total of 100 oral samples were collected from poor oral hygiene patients. Biochemical test characterization such as colony morphology, colour, texture, indole, methyl red, citrate etc. was determined to identify bacteria followed by antibiotic susceptibility test. Sabouraud Dextrose Agar (SDA) culture and Candida Differential Agar (CDA) test were also performed to identify candida species followed by antifungal susceptibility test was performed.