Potato virus X (PXV) is one of the viruses infecting the potato plant, which requires the use of specific methods for its isolation and biological properties. In the research carried out in this direction, a "normal" isolate was isolated from Gala and Diyora potato varieties, and a "necrotic" isolate was isolated from Umid variety, and the characteristics of these isolates were studied and identified by molecular genetic method. To study the biological characteristics of the virus, both isolates were inoculated into several indicator plants, as a result, both isolates were Chenopodium quinoa, Ch. amaranticolor and Ch.mural in plant species yellow chloritic spots, in Gomphrena globosa plant red ring necrosis, in D. stramonium plant, the normal isolate of the virus causes dark systematic mosaic, and the necrotic isolate in this plant causes shrinking of the tips of the leaves and necrotic symptoms in the leaf plate was distinguished by its output. The method of immunoblotting on nitrocellulose membranes was used to determine the natural reservoir plants of the virus, and as a result, a number of plants that were unclear at the initial examination were re-examined, and the virus was identified as Atriplex micrantha C.A. May, Solanum nigrum, Chenopodium murale,. New natural reservoir plants of the virus, such as Ch.quonea, Amaranthus retroflexus, Artemisia annua, A. vulgaris, Sinapis arvensis L., Brassica juncea (L) Czern, which were excluded from IFA refreshment, were identified. In the molecular-genetic identification of the virus, ORFs5 (CP) gene was used as a basis, and as a result, it was confirmed that both isolates were specific for PVC as a result of PCR analysis, and a phylogenetic tree was built based on the sequence of the nucleotide sequence, and the necrotic isolate was placed in the NCBI database under the number MN702769. From the phylogenetic tree compiled on the basis of Blastn bioinformatics programs, it was found that the necrotic isolate of PVC isolated in Uzbekistan was 97.48% similar to the Dutch isolate D00344.1, Australian isolate GU384732.1, and AY297843.1, AY297842.1 and M95516.1 isolated from Great Britain. and isolates were found to share 97.06% homology, suggesting their descent from a common ancestor.