Immunostimulatory agents are the one which stimulate the immune system and provides protection against various infectious diseases.Polysaccharides have proved to have beneficial role in the treatment of many pathological conditions.N-Acetyl D-glucosamine(NAG) is a monomer of polysaccharide,Chitin.The main aim of current work is to study immunostimultory effect of NAG on RAW264.7 macrophages,as the macrophages are the first cells which recognises the pathogen that cause infections.To this macrophages various test doses of NAG were administered during the study period and allowed to observe various parameters that support immunostimulatory effects.Results were obtained from triplicates were statistically analysed for significance.