Aim: This contemplate aimed to compare the impact of normal tap water with ozonated one on both physical and mechanical criteria of acrylic resin denture base material after 7 and 30 days. Materials and Methods: Acrylic resin specimens were fabricated following the ADA specifications No.12 for assessing the surface roughness, hardness, water sorption and solubility which took place after both one week and one month of soaking the acrylic resin specimens in the ozonated water (Group I) and tap water (Group II). Data was statistically analyzed utilizing both independent and paired t-tests.