Background: Healthcare acquired infections (HCAIs) otherwise call nosocomial infection is associated with increased morbidity and mortality among hospitalized patients and predisposes healthcare workers (HCWs) to an increased risk of infections. Healthcare workers (HCWs)-associated infections lead to considerable morbidity, health-care workers infections contribute to increase of higher mortality and higher health-care costs. Prevention and control of infections among primary health-care workers is a critical public health concern. This study assessed Impact of the awareness and practices of infection control standard among health care workers at the primary health are level in Makkah City at Saudi Arabia 2022. Healthcare workers have lost their lives in significant numbers in the discharge of their duties as a result of a breach in Infection Prevention and Control (IPC) procedures. The increasing incidence of emerging and re-emerging diseases complicates this burden. Aim of study: To Assessment the knowledge and practices of infection control standard among health care workers at the primary health are level in Makkah City at Saudi Arabia 2022. Methods: This cross sectional study included (200) health care professionals in Makkah City at Saudi Arabia 2022. (doctors, nurses, lab workers) from primary healthcare (PHC) centers an self-administrated questionnaire was constructed by the researcher and was used for data collection. Divided in to 3 parts and contains 29 items i.e., socio‑demographic characteristics, knowledge questions about infection control and statements about practice of health care providers regarding infection control. Results: there were 200 participants, shows the majority of participant (62.0%) have high of the Knowledge about standard precautions of infection control, followed by (27.5%) of participant average while Range(4-16) and Mean ±SD(11.945±2.981), while regarding the practice the majority of participant (38.0%) have high of the practice about standard precautions of infection control, followed by (36.0%) of participant average while weak were(25.5)while Range(1-13) and Mean ±SD(8.415±2.762). Conclusion: Gaps have been identified in awareness and practice of infection control among health care worker hence, it will be beneficial for all HCW to receive formal and periodic refresher trainings.