Background: Patients with epilepsy are often required to take antiepileptic drugs (AEDs) for a long period of time. Many studies showed that AEDs have a negative impact on the endocrine system in both pediatric and adult populations, including thyroid function, fertility, sexuality, and bone health. Aim: To investigate the effects of traditional antiepileptic drugs (AEDs) versus newer AEDs versus both on the thyroid hormone profile of children with epilepsy. Methods: This was a cross sectional study that was conducted at Pediatrics Neurology Unit. Group I represents patients receiving traditional antiepileptic drugs (AEDs), Group II comprises patients receiving new AEDs, and Group III comprises patients receiving mixed traditional and newer AEDs group. Thyroid profile was assessed in all patients. All patients and healthy group were subjected to laboratory Investigations including FT3, FT4, TSH, by electrochemilumenance serum level of valproic acid and carbamazipene by a homogeneous enzyme immunoassay technique. Results: Sstatistically significant difference between the studied groups regarding free T3. On doing pairwise comparison, difference is significant between traditional AEDs group and mixed AEDs group. There is statistically significant difference between the groups regarding free T4. On doing pairwise comparison, difference is significant between new AEDs group and mixed AEDs group. There is statistically significant difference between the groups regarding TSH. On doing pairwise comparison, difference is significant between new AEDs group and each other group. Conclusion: Mixed traditional and newer antiepileptic drugs had more thyroid dysfunction than monotherapy with traditional or newer antiepileptic drugs.