Biofertilizers are microorganisms that come from the soil's rhizosphere or root nodules. They enrich the soil by increasing the crop's access to nutrients and they decrease the use of chemical fertilizers, which protects the environment. The two main plant nutrients known as the master essential elements in crop production are nitrogen and phosphorus. Beneficial microbes that can fix atmospheric nitrogen or solubilize phosphorus in the soil are some of the beneficial microbes utilized in biofertilizers. Other beneficial microbes include N2 fixing bacteria. In the current investigation, the rhizosphere of chilies in Anand District yielded fifteen Azotobacter isolates and five phosphate solubilizing fungal (PSF) isolates. All isolates were recognized using morphological, cultural, microscopic, and several biochemical assays. The results of yield parameters revealed that the treatment, 100% RDF + commercial Azotobacter + commercial PSF, showed the highest number of fruits (41.67/ plant), number of flowers (55.67 / plant), length of fruit (9.60 cm) and green Chili yield (25.23 t/ ha) was on par with treatment, 100% RDF + Efficient Azotobacter + Efficient PSF i.e., number of flowers (52.87 / plant), number of fruits (39.00 / plant), length of fruit (8.80 cm) and green chili yield (21.69 t/ ha). Whereas, highest dry matter weight (102.21 g), available N (178.75 Kg/ha), P (22.03 Kg/ha) and K (187.22 Kg/ha) was showed by treatment, 100% RDF + commercial Azotobacter + commercial PSF which was on par with treatment, 100% RDF + Efficient Azotobacter + Efficient PSF i.e., dry matter weight (98.00 g), available N (176.66 Kg/ha), P (21.59 Kg/ha) and K (186.40 Kg/ha) in soil after harvest.