This review article explores the challenges and benefits of implementing evidence-based practice guidelines for laboratory testing in nursing care. The article discusses the importance of evidence-based practice in ensuring high-quality patient care and the role of laboratory testing in diagnostic decision-making. Various barriers to the implementation of evidence-based guidelines in nursing practice are identified and strategies to overcome these challenges are discussed. Additionally, the article highlights the impact of evidence-based laboratory testing on patient outcomes and healthcare costs. Overall, this review aims to provide insights into the best practices for incorporating evidence-based guidelines into nursing care settings.