In silico approach is one of the most powerful techniques to discover novel ligand for proteins of known structure and thus play key role in structure based drug discovery. Hence, the purpose of the present study was to perform an in silico docking of nephroprotective properties of phytocompounds identified from Cathranthus roseus leaves namely Octadecanoic acid, Methyl ester, 9-Octadecenoic acid, 9,12-Octadecadienoyl chloride and Hexadecanoic acid against p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun amino terminal kinase (JNK) using Autodock software. Present study concluded that the selected bioactive compounds can efficiently bind to the receptors and molecular docking can be successfully used in finding p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun amino terminal kinase (JNK) inhibitors. Among the various compounds, 9,12-Octadecadienoyl chloride and 9-Octadecenoic acid has potential binding interactions than other compounds. Hence, the studied bioactive compounds have the potential and may be used as nephroprotective agents against kidney diseases. However, these results are only preliminary screening just to facilitate subsequent in vitro and in vivo studies and thus warrants further investigation. To the best of our knowledge, this is the first report that bioactive compounds from hydro-ethanolic extract of Cathranthus roseus are subjected to molecular docking for screening for their nephroprotective potentials.