Drugs used in the treatment of inflammatory bowel diseases (IBD) without side have not developed yet. Based on this, one of the urgent problems is the identification of natural biologically active compounds without side effects that have the property of treating and preventing inflammatory bowel diseases. For the treatment of IBD such as ulcerative colitis and Crohn's disease, Janus kinase(JK) and 5-lipoxygenase (5LO) inhibitors are widely used, but unfortunately, when treated with these drugs, their side effect were observed. Based on this, one of the urgent problems is the development of therapeutic agents without side effects. This article aimed at studying the effect of some biologically active substances isolated from medicinal plants on the activity of 5LO and JK in order to identify drugs with anti-inflammatory properties in the treatment of IBD compared with synthetic drugs such as mesalazine, sulfasalazine, tofacitinib, using in silico methods. These studies show that rutin, dehydroquercetin, quercetin and beta caryophyllene have the highest activity in binding to the enzymes 5LO and JK compared to synthetic drugs.This resultsprovides a basis for further in vitro studies of these compounds to identify anti-inflammatory drugs against IBD.