Numerous diseases, including cancer, have been linked to inflammation, and the treatments that are currently available have side effects. The anti-inflammatory properties of members of the family Amaranthaceae have been demonstrated. The effectiveness of Amaranthus caudatus's in-vitro anti-inflammatory and antioxidant properties was evaluated in this study. This study looked into the seed ethanolic extract of Amaranthus caudatus's phytochemical, antioxidant, and anti-inflammatory properties. Various chemical tests for phytochemical components were performed on the extract; The in-vitro DPPH radical scavenging activity assay was used to measure their antioxidant activity, and the protein denaturation inhibition method was used to measure their anti-inflammatory activity. Carbohydrates, alkaloids, flavonoids, tannins, and phlobatannins were found in extracts after phytochemical testing. Anti-inflammatory studies showed that diclofenac effective concentration (EC50) of 275.19 μg/ml which was higher than EC50 of ethanolic seed extract of 518.92 μg/ml. The effective concentration (EC50) of ascorbic acid was 288.75 μg/ml, which was lower than the EC50 of ethanolic seed extract of 114.25 μg/ml. This study revealed that ethanolic seed extract of Amaranthus caudatus possess anti-oxidant activity against free radicals scavenging in DPPH method and can act as an effective anti-oxidant. The anti-inflammatory effects that inhibit the protein denaturation method