In this study, the role of Clitoria ternatea, Cucurbita maxima, and Artemesia vulgaris extract(s) on remyelination in the prevention of demyelinated disorder such as Multiple sclerosis has been carried out. In-vivo and in-vitro investigation has been done to assess the defensive impact of ethidium bromide-induced demyelination on the rat model. The methanol and petroleum ether extracts of the plants were assessed for anti-inflammatory and antioxidant activity on neuronal cell lines and the assessment of protective effect extracts was conducted on an ethidium bromide induced rat model. Results disclosed that the Cucurbita maxima petroleum ether extract has a substantial influence on ethidium bromide-induced demyelinated rats due to the existence of fatty acids.