Diabetes mellitus is one of the major health problems in the world, the incidence and associated mortality are increasing. Inadequate regulation of the blood sugar imposes serious consequences for health. Conventional antidiabetic drugs are effective, however, also with unavoidable side effects. On the other hand, medicinal plants may act as an alternative source of antidiabetic agents. Here, the presence research investigates the in-vitro and phytochemical effect of ethanolic extract of Allium humie and origanum vulgare for antidiabetic activity. Allium humie belongs to family Alliaceae, is rich in sulfur containing compounds with distinct biological activities. The phytochemical analysis showed the presence of alkaloids, flavonoids, steroids and terpenoids.. The phytochemical test of O. vulgare ethanol extract was showed some compounds, such as a flavonoid, alkaloid, triterpenoid/steroid, essential oil, and tannin. In-vitro assays demonstrated that the extract inhibited α-glucosidase activity, promoted glucose uptake, inhibited glycosylation and relieved oxidative stress, which suggested that O. vulgare extract has a strong hypoglycemic capacity.