Various diseases are mainly caused by the formation of free radicals, and to counter this, various antioxidants are being used now. The focus of the research is to evaluate the antioxidant activity of the plant extract of Dryopteris juxtaposita, which belongs to the Polypodiaceae family. The total phenolic and flavonoid content are also mentioned in this study. Methods used for antioxidant activity were DPPH assay, FRAP (Ferric Reducing Antioxidant Power) assay, hydroxyl radical scavenging activity, and TAC (Total antioxidant capacity) assay. For the estimation of flavonoids and phenols, aluminium chloride and Folin–Ciocalteu reagents were used, respectively. Two parts of Dryopteris juxtaposita (leaves and stem) were used, among them leaves shown high antioxidant activity then stem. In DPPH assay, leaves were inhibiting 93.36 % of free radicals and in hydroxyl radical scavenging activity it were inhibiting 73.08 % of free radicals at 100 μg/ml concentration. While stem shown 81.17% and 70.59% inhibition of DPPH and hydroxyl radical at same concentration respectively. TAC by phosphomolybdenum reagent and FRAP assay depicted higher antioxidant values in leaves then stem. The total phenolic content was recorded higher in stem than leaves, i.e., 397 mg GAE/g and 159.5 GAE/g respectively whereas, total flavonoids content was 33.66 mg QA/g and 6.06 mg QA/g in leaves and stem respectively. The methanol and water (7:3) extract of Dryopteris juxtaposita was recorded very good natural antioxidant activity having high content of phenolic and flavonoid compounds while comparing with marketed available standard Antioxidant. So, it can be used as an alternative for various diseases caused by free radical.