Ojective: The current research work was at attempt to investigate the in-vitro antioxidant activity of diosmin loaded phytosomal preparation by using DPPH Assay. Method: Phytosome was prepared with Diosmin and Phospholipid by using solvent evaporation method. The in-vitro antioxidant activity was measured using DPPH method for evaluation of FRSA. Results: the outcome of the research work clearly indicated that Diosmin loaded Phytosmal formulation replicated better free radical scavenging activity(FRSA) and results showed 78.21±0.82 % with Diosmin and 85.11±0.24% with phytosome at Highest concentration (80µg/ml)and IC50 of Diosmin was 43.15 µg/ml and IC50 for Diosmin Loaded Phytosome was 39.01 µg/ml. Conclusion: Experimental results showed that Diosmin Phytosome has significant Antioxidant Activity.