Papaya (Carica Papaya Linn.) is well known for its exceptional nutritional and medicinal properties through the world. The whole papaya plant including its leaves, seeds, ripe and unripe fruits and their juice are used as a traditional medicine. Now a days papaya is considered as a nutraceutical fruit, due to its multi – faceted medicinal properties. The papaya (Carica Papaya.,L) seed contains fatty acids, crude protein, benzyl isothiyocynate.It does not contain any heavy metals such as lead and arsenic. Papaya seed powder incorporated choco chip cookies was standardized and formulated in three variations. All the recipe variations were subjected to sensory evaluation by the panel members. The mean score of acceptability of the selected recipes with the incorporation of various level of papaya (Carica Papaya.,L) seed powder revealed that the variation 1 (V1 ) 5 per cent had the highest level of acceptability among the other variations of the papaya (Carica Papaya.,L) seed powder incorporated choco chip cookies. The papaya (Carica Papaya.,L) seed powder cookies from variation 1 (V1 ) had the highest acceptability in sensory attributes like appearance of (7.8), colour (7.5), flavour (7.8), taste(7.4), texture(7.2) and overall acceptability(7.4) among all the variations. The seed incorporated cho – co chip cookies contained anti-cancer property. It shows that it has an IC – 50 value of 92.37% µg/ml which denoted the anti cancer activity