MicroRNA – a key regulator of gene expression which are conserved across species. The regulation of miRNA biogenesis was influenced by precursors and regulated by their subsequent mature miRNA processing. These protein regulators are bound to the mature miRNA that directs degradation and preventing their expression. Most common regulatory protein is Lin28, which will bind let-7 miRNA and its target degradation. Cancer –a genetic disease, involves changes in structure and gene expression. The main mechanism of cancer cells is to attack and destroy normal cells due to body imbalance and can be treated by correcting the unevenness. A change in expression implicated miRNA down regulation in cancer cells and reintroducing miRNAs in animals have impaired cancer cell viability and cell death. Researchers in worldwide validated “miRNA replacement therapy” theory involves introduction of either miRNAs mimetic or synthetic miRNA in affected tissues, to restore the normal proliferation, cell cycle, apoptosis and cellular mechanism, which makes pharmaceutical industry to develop therapeutic and diagnostic targets. This study aimed to check miRNA stability and miRNA modulations on treatment with phyto- lipids isolated from medicinal plants. Calculated cell viability percentage showed increased cell stability in lipid treatment samples indicating the longevity property exhibited by the lipid extracts. In-vitro cytotoxicity assay on Breast cancer cell line MDA MB 231 treated with lipid extracts dissolved in DMSO, revealed the anti-cancerous property of Alpinia galanga exhibited at low IC50 Value of 774.3 μM/ ml.