To test N-Acetyl-D-Glucosamine in vivo antimicrobial activity against Salmonella typhimurium -induced infectious rat model. Methods: A Salmonella typhimurium-infected rat model was used in the present study to evaluate the anti-microbial activity of NAG. The effects of NAG on infected Wistar rats were studied by using standard methods. Changes in various parameters such as weight gain, water intake, hematological parameters, lipid profile, and histopathology upon administration of NAG were studied. Results: It has been demonstrated that N-Acetyl-D-Glucosamine has a significant effect on the number of viable Salmonella typhimurium recovered from feces and could be able to stop salmonellosis after 8 to 10 days of treatment in male Wistar rats. When compared to the positive control group, NAG had a significant effect on restoring healthy body weight, individual organ weights, hematological parameters, and histopathological changes in liver tissue. Conclusion: The overall results demonstrated that NAG has the potential to provide an alternative effective treatment against salmonellosis, including typhoid