This research's objective is to examine and assess the cytotoxicity and genotoxicity of prazosin HCL in pregnant mice. Prazosin (PZ) was administered to the animals intraperitoneally (IP) at dosages of 5, 15, and 25 mg/kg/body weight for single dose (14-day) toxicity tests. The investigation was conducted using a variety of techniques, including estimations of the levels of reduced glutathione (GSH), Malondialdehyde (MDA), body weight, organ weight, and food intake. The following parameters have been examined for evaluating genotoxicity: DNA fragmentation assay for determining DNA damage, metaphase chromosomal analysis. The collected information conclusively demonstrates that PZ was harmful to hepatocytes at the higher dose as indicated by elevated MDA levels, decreased GSH levels, DNA damage, and elevated DNA fragmentation in pregnant mice. It's also intriguing to see that PZ caused considerable DNA strand breaks and structural chromosomal abnormalities in bone marrow cell lines. Therefore, it is regarded as being genotoxic to mouse hepatocytes and bone marrow cells. The current investigation demonstrated that Prazosin, at its corresponding hepatotoxic dose level, caused severe genotoxic effects in pregnant mice.