The objective of present study was to develop rectal formulation of based on microparticles and perform in vivo study of developed rectal formulation in rats. The formulated suppositories were examined for different evaluation parameters like weight variation, disintegration time, in vitro dissolution study, stability study and pharmacokinetic study. The disintegration time and percent cumulative drug release of the suppositories were determined in the range between 13.69±0.93 min to 20.94±0.63 min and 88.23±0.91 to 96.47±0.02 respectively. During In vivo pharmacokinetic study in male Sprague–Dawley Rats, the relative bioavailability of rectally administered felodipine microparticles loaded suppository was found 148.15%. The study reveals that rectal administration of felodipine as microparticles loaded suppository was an alternate route of administration.