Abelmoschus esculentus L (Malvaceae ), commonly named Lady finger is one of the commonly used medicinal plants. Historically it is found that stem of A. esculentus L contain a variety of compounds that have been linked to diabetes mellitus. Okra stem contained the greatest concentration of phenolic and flavonoid compounds. Okra had a lot of fibre. Objective: The present study was carried out to investigate the traditional use of Abelmoschus esculentus L stem in alloxan monohydrate -induced diabetes in rats alongwith antidiabetic chemical constituents in stem and was tested for in vivo biological activities. Methods: The ethanolic, aqueous and chloroform extract was obtained by the Reflux extraction method, and fractionation was done with column chromatography and TLC. The antidiabetic chemical constituent has been confirmed by Gas Chromatography-Mass Spectroscopy (GC-HRMS) analysis. For in vivo activities, rats with diabetes mellitus caused by alloxan monohydrate were selected and the anti-diabetic indicators assessed were body weight, blood glucose level, insulin and urine volume. Results: Fraction of the Chloroform extract showed presence of Lysine, Leucine, Dodeanoic acid, Pentanoic acid in GC-HRMS Study. In GC-HRMS analysis determine Leucine is present in highest area of 2565054.20 with molecular weight 265. This Chemical constituent decreased blood glucose levels and has positive effects to cure diabetes. The extracts and fraction with oral dose were compared with standard drug metformin (150 mg/kg b. w). A.esculentus chloroform extract Fraction (AECEF) showed better effect than other extracts used for study. Conclusion: From the results we can conclude that Abelmoschus esculentus L plant, is having various antidiabetic compounds with effective activity