Background: Bisphenol A (BPA) is an organic compound that is widely used in the production of plastic substances. It is thought to play a role in the pathogenesis of many diseases, including Polycystic Ovary Syndrome (PCOS). Aim: Determination of the association between urinary BPA level and PCOS in Egyptian females.. Subjects and methods: The current study included 120 female subjects, 60 patients diagnosed with PCOS based on Modified Rotterdam's Criteria and 60 healthy controls. All included females were subjected to full history taking, clinical examination and pelvi-abdominal ultrasound examination. Panel of laboratory assay included FSH, LH, fasting blood glucose, fasting insulin level, HOMA-IR, DHEAs and free testosterone. Urinary BPA was measured in all subjects using UHPLC in-house method. Results: Body mass index was significantly higher in PCOS than control group (p value <0.01). Regarding laboratory findings, LH, LH/FSH ratio, HOMA-IR, DHEAs and free testosterone all showed significantly higher levels in PCOS compared to healthy controls. Urinary BPA was significantly higher in PCOS than control group whether adjusted to urinary creatinine or not with p values 0.04 and 0.17 respectively. Conclusion: Levels of BPA were significantly higher in PCOS cases than controls.