This experiment was carried out in the Laboratory of Postgraduate Studies / Biotechnologies / College of Science / University of Kufa / for the period from 10/1/2022 to 1/ 4/2023.This study was conducted to extract the essential oils and the crude extracts from the seeds of the Mentha spicata medicinal plant. The essential oils were extracted from the fresh leafs of plant using the Clevenger hydro-distillation method and oils were detected using sulfuric acid reagent. Study the solubility, acidity and the molting point of these essential oils. Also, The basic chemical compounds of the seeds crude extracts were investigated and detected using some chemical reagents for alkaloids, phenols, and glycosides, as well as terpene compounds, and essential oils were detected using the sulfuric acid detector and the filter paper method. Gas chromatography (GC-MS) methods were using to determine chemical compounds of the essential oils and study their biological activities against some bacteria that cause gingivitis isolated from infected male gums. Four concentrations of Oils and alcoholic seeds crude extract were tested 1:1, 2/1, 5/1, 10/1 (v/v) ml of oil /ml of distilled water, in addition to the standard substance as menthol( S1) as a control agent, and Crude Oil as standard (S2) and Kanologe drugs as Positive control (P ⃰ ) to evaluate the antibacterial activity that causes gingivitis, using the agar diffusion method, at a rate of three replicates for each concentration and 9 replicates for each treatments . The bacterial samples that cause gingivitis were obtained from Al-Ameen Center for Research and Advanced Biotechnology in Al-Najaf City . The diagnosis of bacteria isolate was also confirmed with the Vitek2 Compact. The results showed that there was a conformity with the ideal specifications of the oils with international standards or close to them, where the solubility 99% in ethanol solvent compare with Methanol , ethers acetone , water was recorded( 98, 88,4 )% respectively and the boiling point is 98 ° C ,Acidity 2.89n. The results of the qualitative detection of essential oils were positive with sulfuric acid reagents, and filter paper reagents. Also, The results of the qualitative detection of alcoholic extracts of seeds were positive with the studied chemical reagents, except for the detection of alkaloids, which showed negative results. The number of chemical compounds of essential oils were identified about 31 compounds with different percentages. Menthol was the highest at 21.21%, followed by Thymol at 12.53% and Thujone at 4.01%. The results showed that all the concentrations used gave an area of bacterial inhibition growth rate that increased with increasing oil and seeds extract concentration. The highest inhibition rate of p.areuginosa was for oils at a concentration of (1/10) v/v ml Oils: water , with an average inhibition rate of (20 )mm , and that the highest inhibition rate was for seeds extracts at a concentration of (1/10) v/v ml extract : water , with an average inhibition rate of (22) mm. While the lowest inhibition rate was for the Oils and seeds extracts at a concentration of (1/1) v/v ml Oils :water with an average inhibition of ( 00 and 16) mm respectively . However, The highest inhibition rate Enterococcus fecalis bacteria was for oils at a concentration of (1/10) v/v ml Oils:water , with an average inhibition rate of (30) mm, and that the highest inhibition rate was for seeds extracts at a concentration of (1/10) v/v ml extract : water , with an average inhibition rate of (20) mm While the lowest inhibition rate was for the Oils and leafs extracts at a concentration of (1/1) v/v ml Oils :water with an average inhibition of ( 10 and 20) mm respectively